Study shows higher relation between collagen I and II after autologus stem cell treatment. New Zealand White rabbitCollagen is the main supportive protein of cartilage, connective tissue, tendon, skin, and bone. It is a fibrous insoluble protein consisting of bundles of tiny reticular fibrils that combine to form the white glistening inelastic fibers of the tendons, the ligaments, and the fascia.

Alex Vaisman, MD, at 8th World Congress of the International Cartilage Repair Society presentation, reveled that collagen scaffold along with mesenchymal stem cells compromised to the chondrocyte lineage and platelet rich plasma shows promising results in treating full thickness chondral defects.

Vaisman and his team conducted the research with adult male New Zealand White rabbits, they created 20 mm2 acute full-thickness chondral defects in 36 femoral condyles of the subjects.

The research team randomly assigned the rabbits to the following four groups:

* Group 1 in which the lesion was left untreated;
* Group 2 in which surgeons implanted a scaffold without MSCs or PRP;
* Group 3 in which a scaffold containing MSCs was implanted; and
* Group 4 in which a scaffold contained MSCs and PRP.

After six months researcher evaluated the femoral condyles macroscopically, histologically rabit-knee-with-scaffoldusing hematoxylin-eosin and Toluidine Blue staining, and molecularly using quantitative real-time polymerase chain reaction of Collagen II/I and aggrecan/versican.

Investigators discovered:

  • fibrous tissue without bony exposure in the control group.
  • Groups 2 and 3 showed a hypertrophic, soft, irregular tissue covering the whole lesion.
  • Group 4 has similar- to normal-hyaline cartilage.

Through molecular analysis researchers identified significantly higher relation between Collagen II and I in group 3 and 4 compared to others.

Source: Ortho Supersite

For further information regarding the study please contact:

*   Alan J. Nixon, MD, can be reached at Cornell University, College of Veterinary Medicine, C3-187 VMC, Ithaca, NY 14853; 607-253-3224; e-mail:

* Alex Vaisman, MD, can be reached at Orthopaedic Surgery Unit, Clínica Alemana de Santiago, and Faculty of Medicine, Universidad del Desarrollo, P.O. Box 3737, Santiago, Chile; (56-2) 210-11 11, 212-97 00; e-mail: They have no direct financial interest in any companies or products mentioned in this article.