A India Scientist Hinduja Zavari is one of the first scientists to have created embryonic stem cell lines without using mouse feeder cells to grow them. Indira Hinduja, who created the country’s first test-tube baby, used human feeder cells to grow the embryonic stem cell lines so they would not be contaminated.
Hinduja and IVF specialist Kusum Zaveri developed three human embryonic stem cell lines without using the contaminating cells to grow them as most researchers have done around the world, according to a Chennai Online news report.
Using the human feeder cells to grow the lines will also help the embryonic stem cells to be able to be reimplanted up to 200 times as opposed to the limit of 75 times using mouse feeder cells.
Embryonic stem cells are normally maintained on mouse embryonic fibroblast (MEFs) feeder layers but the India announcement is the first from scientists saying they created embryonic stem cell lines without mouse feeder cells.