Hematopoietic stem cells (HSCs) are one of the few cell types able to resist infection with HIV-1 despite expressing the cell surface molecules to which HIV-1 binds before entering a cell.
In a study that appears in the February issue of the Journal of Clinical Investigation, researchers from Harvard Medical School, Boston, show that HSC expression of a protein known as p21Waf1/Cip1/Sdi1 (p21) is required for HSCs to be resistant to infection with HIV-1.
David Scadden and colleagues showed that HSCs in which expression of p21 was decreased were more susceptible to infection with HIV-1 than cells expressing normal levels of p21.
Further analysis showed that p21 did not inhibit HIV-1 entering the cells, rather it prevented the viral DNA integrating into the host cell genome by binding to the HIV-1 integrase complex and preventing it from mediating chromosomal integration. This protective mechanism was specific for HIV-1, as decreased expression of p21 in HSCs did not allow a related virus (SIVmac-251) to productively infect the HSCs.
This study therefore identifies p21 as a protective factor that prevents HSCs being infected with HIV-1.
Source:Journal of Clinical Investigation [www.jci.org/cgi/content/full/117/2/473?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=Protein+Stops+HIV-1+In+Its+Tracks+In+Hematopoietic+Stem+Cells&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT]